Fig. 5.
Fig. 5. Blocking of cytotoxicity mediated by NT4.2 cells against auto LCL cells, allo LCL cells, and auto PHA-blasts. A total of 5 × 104 NT4.2 cells were incubated with 5 × 103 51Cr-labeled target cells in medium (Med) alone or with addition of the indicated MoAbs: anti–HLA-DR, DQ, DP, anti-HLA class I, and anti-CD3, ascites 1/100; anti-HLA DR2, purified MoAb 10 mg/mL. The mean percentage of specific lysis was determined from triplicate cultures after 4 hours of incubation.

Blocking of cytotoxicity mediated by NT4.2 cells against auto LCL cells, allo LCL cells, and auto PHA-blasts. A total of 5 × 104 NT4.2 cells were incubated with 5 × 10351Cr-labeled target cells in medium (Med) alone or with addition of the indicated MoAbs: anti–HLA-DR, DQ, DP, anti-HLA class I, and anti-CD3, ascites 1/100; anti-HLA DR2, purified MoAb 10 mg/mL. The mean percentage of specific lysis was determined from triplicate cultures after 4 hours of incubation.

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