Western blot analysis of recombinant and plasma fibrinogens. Samples were run on a 10% SDS gel under reduced conditions. Lanes 1, 3, 5, and 7 were recombinant fibrinogen and 2, 4, 6, and 8 were plasma fibrinogen. The primary antibody for lanes 1 and 2 was a polyclonal antiserum raised against human fibrinogen (Dako), for lanes 3 and 4, a monoclonal antiserum that is specific for the N-terminus of the Aα chain,19 for lanes 5 and 6, a monoclonal antiserum specific for the N-terminus of the Bβ chain,20 and for lanes 7 and 8, a monoclonal antiserum specific for the C-terminus of the γ chain.21 Molecular weight markers are indicated at the right.