Fig. 6.
Effects of BSO on (A) LPS induced increases in GSH and (B) LPS-induced blockade of anti-Fas IgM induced neutrophil apoptosis. Neutrophils (1 × 106/mL) were preincubated with or without BSO (50 mmol/L) and then incubated with LPS (1 μg/mL) or LPS (1 μg/mL) + GSH (25 mmol/L), all groups were incubated after 1 hour with or without Fas (100 ng/mL). Intracellular GSH and apoptosis were assessed at 1 hour and 18 hours, respectively. *P < .05 versus control. Results are the mean ± SD for n = 3 experiments with three separate donors of neutrophils.