DNA-binding specificity of the p45/MafG heterodimer. An NF-E2 DNA-binding site derived from the human PBGD promoter was used as a probe to detect NF-E2 DNA binding activity in nuclear extracts from COS-7 cells cotransfected with expression constructs coding for p45 NF-E2 and MafG. A series of wild-type and mutant NF-E2 binding site oligonucleotides were used as competitors. The sequences of competitor oligonucleotides 1 to 16 have been described previously.16