Unsorted peripheral blood cells after FISH with a chromosome 12-specific α satellite DNA probe conjugated to spectrum orange. Three fluorescent hybridization signals indicate trisomy 12; two signals represent diploid cells (left). The right side shows a sorted CD34⁺/38⁺ cell hybridized to a spectrum green labeled a chromosome 12-specific α satellite DNA probe. The three hybridization spots indicate trisomy 12 (right).