DNase I footprinting analysis of the type 2 CD10/NEP promoter. The 314-bp fragment extending from bp −208 to +107 of the CD10/NEP promoter was 32P-labeled on the noncoding strand (A) or coding strand (B) and incubated with no nuclear protein (free, lanes 2 and 6), 100 μg Nalm-6 nuclear protein (lanes 3 and 7), or FHTE56 nuclear protein (lanes 4 and 8). Molecular weight markers (lanes 1 and 5) were generated by G+A-specific Maxam-Gilbert cleavage. Numbers at the left of each panel indicate nucleotides upstream(−) or downstream(+) of the major transcription initiation site (+1). Sequences protected from DNase I digestion are indicated by brackets. (C) Sequence of the CD10/NEP promoter and three protected regions. The arrow delineates the major transcription initiation site (+1).24
