EMSA of the type 2 CD10/NEP promoter with wild-type region I (A), II (B), or III (C) probes. Double-stranded oligonucleotides from regions I, II, or III were ³²P-labeled and incubated with 5 μg nuclear protein from Nalm-6 (lanes 1, 2, 5, 6, 9, and 10) or FHTE56 (lanes 3, 4, 7, 8, 11, and 12) in the absence (−) or presence (+) of 100-fold molar excess unlabeled self-oligonucleotide. Arrows identify the major shifted bands.