EMSA of the type 2 CD10/NEP promoter with mutated region I, II, or III probes. (A) Sequences of wild-type and mutant region I, II, or III oligonucleotide probes. The 10-bp mutations in region I, II, and III oligonucleotide probes are indicated. (B, C, and D) EMSAs. A double-stranded oligonucleotide of wild-type (W) region I (B), region II (C), or region III (D) was ³²P-labeled and incubated with 5 μg nuclear protein from Nalm-6 in the absence (−, lanes 1, 5, and 9) or presence of 100-fold molar excess unlabeled W (lanes 2, 6, and 10) or mutated ([M] lanes 3, 7, and 11) oligonucleotide. Double-stranded mutated region I, II, or III oligonucleotide probes (M) were also ³²P-labeled and incubated with 5 μg nuclear protein from Nalm-6 (lanes 4, 8, and 12). Arrows identify the major shifted bands.