Fig. 7.
Fig. 7. Detection of alternatively spliced CBF-B/NF-YA subunits in lymphoid and epithelial cell extracts. Nuclear extracts from Raji and Nalm-6 lymphoid cells and FHTE56 and Calu-1 epithelial cells were size-fractionated, blotted, and incubated with an affinity-purified antiserum directed against the C-terminal peptide of NF-YA.35 The ∼40-kD CBF-B/NF-YA short form and the ∼43-kD CBF-B/NF-YA long form are indicated.

Detection of alternatively spliced CBF-B/NF-YA subunits in lymphoid and epithelial cell extracts. Nuclear extracts from Raji and Nalm-6 lymphoid cells and FHTE56 and Calu-1 epithelial cells were size-fractionated, blotted, and incubated with an affinity-purified antiserum directed against the C-terminal peptide of NF-YA.35 The ∼40-kD CBF-B/NF-YA short form and the ∼43-kD CBF-B/NF-YA long form are indicated.

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