RT-PCR for Fas (CD95) expression in EC. PCR analysis of cDNA reverse transcribed from RNAs obtained from microvascular EC of various tissues and from large vessel umbilical vein and coronary artery EC cultured for 18 hours in the presence of various plasmas (1:100 dilutions) was performed. Simultaneous RT-PCR for β-actin, using aliquots from these same samples, served as a control for integrity of the starting materials and gave equivalent signals for all samples (data not shown). The two bands for Fas recognized in some lanes represent the membrane-bound and secreted forms of this molecule.17 (A) Lane 1, dermal MVEC-2 + normal plasma; lane 2, dermal MVEC-2 + TTP-1; lane 3, renal HMVEC-G + normal plasma; lane 4, renal HMVEC-G + TTP-1; lane 5, renal HMVEC-G + TTP-2. (B) Lane 1, pulmonary HMVEC-LU + normal plasma; lane 2, pulmonary HMVEC-LU + TTP-1; lane 3, pulmonary HMVEC-LU + TTP-2; lane 4, hepatic HMVEC-H + normal plasma; lane 5, hepatic HMVEC-H + TTP-1; lane 6, hepatic HMVEC-H + TTP-3. (C) Lane 1, UVEC + normal plasma; lane 2, UVEC + TTP-1; lane 3, coronary HCAEC + normal plasma; lane 4, coronary HCAEC + TTP-1.