Analysis of hematologic reconstitution after BMT. Spleen cells from (A) B6 mice grafted with FVB BM and (B) B6 mice grafted with FVB BM plus EpTK T cells and treated with GCV were analyzed at different times after BMT. (C) is a B6 control mouse. Three-color flow cytometric analyses were performed with a combination of the B-cell–specific B220 MoAb with Thy1.2 and H-2^b (recipient type) or Thy1.1 and H-2^q (donor type) MoAbs. Monoparametric histograms are depicted in B220⁺ and B220⁻ gated subsets. Data are representative of reproducible experiments performed at days 22, 51, 70, and 212 after BMT.