![Fig. 4. Genomic analysis of CHEMR1 gene. Evidence for existence of the human homologue of CHEMR1 and for CHEMR1 being intronless. (A) 129/J genomic DNA (20 μg) and human genomic DNA prepared from Mo7e cells were digested with the restriction enzymes indicated, run on a 1% agarose gel, transferred to Quiabrane, and hybridized with [32P] CHEMR1 ORF. The filter was washed finally with 0.5× SSC, 0.1% SDS at 65°C, and the blots were exposed to XAR-5 film in cassettes at −70°C for 6 days. DNA molecular standards from BRL were run in parallel. The CHEMR1 cDNA has two Kpn I (−26 and 840) and HincII (580 and 1,110) sites, which release around 867 and 531 bp, respectively. These bands are indicated by arrows. (B) One microgram of 129/J genomic DNA or OCM1 genomic DNA was amplified with Pfu polymerase in the presence of 50 pmol of each of the corresponding primers as indicated in the Materials and Methods. After 30 cycles of amplification, one-tenth volume of PCR reaction mixture was resolved on a 1% agarose gel. Indicated with arrows are amplified ORFs of chemokine receptors. The same molecular standards were used as in (A).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/12/10.1182_blood.v89.12.4448/3/bl_0032f4.jpeg?Expires=1735565757&Signature=SS4QTwgIpQ6eELKMCUvQilQ-KdUR--p-cqmgZdwXdV6isqAXbq0uXq2yESOIq8m0ryazwpoVZPLmVaR00C8B9eiTYmdUScuNUK85rFBv6yadwgtzLKzIdYdAWcE9liiit7dg7VvEflAC1RV-BJrsa2jffsXBm~xE5SlFUUF1EB5nYEYFOmcAdNpttI6zzxneXCYa4pOqo5ywjHzERglqo-M0C0ruGAnHEphLHtF5iMLkLFmuw~n3V~xMIXRbXbXj30ETCtwxfR2fVhZeu2gbGv5~G4MpkGXXaZLTLhg7RZiz6-Ov32gSx85m4VG9RkDbCVNXsMN9ybZzV15YM1sDKw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Genomic analysis of CHEMR1 gene. Evidence for existence of the human homologue of CHEMR1 and for CHEMR1 being intronless. (A) 129/J genomic DNA (20 μg) and human genomic DNA prepared from Mo7e cells were digested with the restriction enzymes indicated, run on a 1% agarose gel, transferred to Quiabrane, and hybridized with [32P] CHEMR1 ORF. The filter was washed finally with 0.5× SSC, 0.1% SDS at 65°C, and the blots were exposed to XAR-5 film in cassettes at −70°C for 6 days. DNA molecular standards from BRL were run in parallel. The CHEMR1 cDNA has two Kpn I (−26 and 840) and HincII (580 and 1,110) sites, which release around 867 and 531 bp, respectively. These bands are indicated by arrows. (B) One microgram of 129/J genomic DNA or OCM1 genomic DNA was amplified with Pfu polymerase in the presence of 50 pmol of each of the corresponding primers as indicated in the Materials and Methods. After 30 cycles of amplification, one-tenth volume of PCR reaction mixture was resolved on a 1% agarose gel. Indicated with arrows are amplified ORFs of chemokine receptors. The same molecular standards were used as in (A).
