Fig. 6.
![Fig. 6. Northern blot analysis of HEK293 cells transfected with vector (pRc/CMV), the antisense CHEMR1, sense CHEMR1, and CCR-1 in pRc/CMV. HEK293 cells were transfected with 20 μg of each DNA using the modified calcium phosphate method. After 24 hours, total RNA was isolated with Tri-reagent (Molecular Research Center, Cincinnati, OH). Twenty micrograms of the RNA was run on a 1.2% formaldehyde gel, blotted to Quiabrane, and hybridized with [32P] CHEMR1 cDNA. The filter was washed finally with 0.5× SSC, 0.1% SDS at 65°C, and the blot was exposed to XAR-5 film in a cassette at −70°C for 2 days. The same filter was stripped and subjected to sequential hybridization with [32P] CCR-1 and [32P] rat GAPDH probe, respectively.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/12/10.1182_blood.v89.12.4448/3/bl_0032f6.jpeg?Expires=1735566230&Signature=1EvbcksGPdKOeCYW9-Z19wlGJevqrwRRKi~l8WvF5LyQBYAmHOV-CSMpJEmZhPdct1eayhbM3pa25xSghAfeUInxweVd8EYBovqpFKGCr4ujZ1fCM451QGetJ1UOtuB6qZlhk7t9Og2s~3TuHGfRCSjGW25Uq5ao8ux9xHlp0kzvCqRHGPMZDQQHfpPrmxTuPPQpEYVAI6Q-92-et9PCfws-4pgcj2HqUxQeyQLiDWfAY1MA~h308~OySvLzcsGXOKiZh92nQJJ4VYcKvpBZFuYBs780n21qa1sJn-sWJzBjBwvN6xiAWjyA-5lli4F5ZDxiq4KVx8U031TxbjjxMQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Northern blot analysis of HEK293 cells transfected with vector (pRc/CMV), the antisense CHEMR1, sense CHEMR1, and CCR-1 in pRc/CMV. HEK293 cells were transfected with 20 μg of each DNA using the modified calcium phosphate method. After 24 hours, total RNA was isolated with Tri-reagent (Molecular Research Center, Cincinnati, OH). Twenty micrograms of the RNA was run on a 1.2% formaldehyde gel, blotted to Quiabrane, and hybridized with [32P] CHEMR1 cDNA. The filter was washed finally with 0.5× SSC, 0.1% SDS at 65°C, and the blot was exposed to XAR-5 film in a cassette at −70°C for 2 days. The same filter was stripped and subjected to sequential hybridization with [32P] CCR-1 and [32P] rat GAPDH probe, respectively.
