Production of eosinophil O−⋅2 by triggering Fc-γ receptors using the spin trapping technique. (A) EPR spectrum of isolated hepatic granuloma eosinophils (2 × 106 cells/mL) suspended in HBSS in the presence of DTPA (0.1 mmol/L) and DMPO (100 mmol/L) after incubation for 45 minutes at 37°C with 75 μg of 2.4G2. (B) EPR spectrum after 25 minutes of addition of opsonized zymosan (1 mg/mL). (C) EPR spectrum of purified eosinophils incubated during 20 minutes with 75 μg of 2.4G2 and then added opsonized zymosan (1 mg/mL) for 25 minutes. (D) EPR spectrum of purified eosinophils incubated for 20 minutes with 75 μg of B3B4 followed by the addition of opsonized zymosan (1 mg/mL) for 25 minutes. (E) EPR spectrum of purified eosinophils preincubated with 40 μg of F(ab′)2 fragments of 2.4G2 and then incubated with 2.4G2 and opsonized zymosan in the same conditions as described in (C).
