Fig. 1.
Inhibition of MLR across major histocompatibility barriers. (A) BALB/c (H-2d) × C57BL/6 (H-2b); (B) BALB/c × PL/J (H-2u); (C) BALB/c × C3H (H-2k). A total of 2.5 × 105 responder spleen cells were plated with 5 × 105 irradiated (30 Gy) spleen cell stimulators in the absence or presence of CD31 peptide or control peptide. After 96 hours of incubation, cultures were pulsed with 1 μCi [3H] thymidine per well for an additional 16 hours. Results are the mean cpm from triplicate cultures and representative of 3 separate experiments.