Fig. 2.
Fig. 2. Inhibition of MLR across minor histocompatibility barriers (B10.D2/nSnJ [H-2d], responder; BALB/c [H-2d], stimulator). This combination is the same as in the in vivo system. 1 × 106 nylon wood–purified responder T cells were plated with 1 × 106 irradiated (10 Gy) spleen cell stimulators in the absence or presence of CD31 peptide or control peptide. After 5 days' incubation, cells were harvested and counted (cultures were pulsed with 1 mCi [3H]thymidine per well for 16 hours before harvest). Results are the mean cpm from triplicate cultures and are representative of 2 separate experiments.

Inhibition of MLR across minor histocompatibility barriers (B10.D2/nSnJ [H-2d], responder; BALB/c [H-2d], stimulator). This combination is the same as in the in vivo system. 1 × 106 nylon wood–purified responder T cells were plated with 1 × 106 irradiated (10 Gy) spleen cell stimulators in the absence or presence of CD31 peptide or control peptide. After 5 days' incubation, cells were harvested and counted (cultures were pulsed with 1 mCi [3H]thymidine per well for 16 hours before harvest). Results are the mean cpm from triplicate cultures and are representative of 2 separate experiments.

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