Fig. 5.
![Fig. 5. 1934.4 hybridoma assay. 1934.4 hybridoma cells (5 × 104) were incubated with 5 × 105 PL/J spleen cells and various concentrations (0.01 to 1,000 μmol/L) of Ac1-11 [4A] for 24 hours in the presence of CD31 peptide (200 μg/mL) or the absence of peptide. The IL-2 production of the Ac1-11 [4A]-reactive hybridoma 1994.4 was used as a readout system for antigen presentation. IL-2 production was measured by proliferation of the IL-2–dependent cell line HT-2 in the presence of supernatants from 1994.4 cultures. Results are expressed as the mean cpm from triplicate cultures. Results are representative of 3 separate experiments](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/4/10.1182_blood.v89.4.1452/4/bl_0014f5.jpeg?Expires=1735835190&Signature=sEouPUs-lp5u0DyUFnIbqgaFtqBrRdCD1iOQMKNpky4JTI92XiCoSXAoT-ejMK0lMwSF77sfSp7ewd-C9qvk1gDTRuDFVCqKbru4dNxCdyJ001PR6t2LZqhjg04emTqiFY37tW2QrkdeH4i0VWq2SZZbzUSmm9szIsep5XVtB7KmJE9vGHzahp6l7k3nHzRbOzkvX0njJ5uoZAgwuO6JGMevgwZFToncPdiura6MXBbRUUtXzgdUDHOBK71PkysNG9Ut2nkfFjEca9ptZG1Qh-PD9Shmh2734FUsqnEobSdzyojZ~6iLUrHr5cLwjX-TWu80D8y3OHbA0yLtxs0Oww__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
1934.4 hybridoma assay. 1934.4 hybridoma cells (5 × 104) were incubated with 5 × 105 PL/J spleen cells and various concentrations (0.01 to 1,000 μmol/L) of Ac1-11 [4A] for 24 hours in the presence of CD31 peptide (200 μg/mL) or the absence of peptide. The IL-2 production of the Ac1-11 [4A]-reactive hybridoma 1994.4 was used as a readout system for antigen presentation. IL-2 production was measured by proliferation of the IL-2–dependent cell line HT-2 in the presence of supernatants from 1994.4 cultures. Results are expressed as the mean cpm from triplicate cultures. Results are representative of 3 separate experiments
