Engraftment of donor bone marrow in the B10.D2 → BALB/c transplant documented by PCR amplification of a polymorphic microsatallite region within the IL-1β gene. Long-term engraftment of allogeneic bone marrow was found in all transplanted mice. This figure demonstrates one representative analysis on an ethidium bromide–stained 1.5% agarose gel. Lane 1, DNA marker φx174 digested with HaeIII (marker sizes: 1,353, 1,078, 872, 603, 310, 281, 271, 243, and 194 bp; GIBCO BRL, Gaithersburg, MD); lane 2, negative control (PCR without DNA); lane 3, recipient (BALB/c) standard; lane 4, donor (B10.D2) standard; lanes 5, 6, 7, and 8, recipients given CD31 peptide (ID no. 669, 670, 672, and 674); lane 9, recipient given control peptide (ID no. 691); lanes 10 and 11, recipients given PBS (ID no. 773 and 774).