Phenotype of cells generated in wells seeded with single CD34⁺CD38^lowCD19⁻ cells. In (A), wells precoated with MS-5 cells were seeded with single CD34⁺CD38^lowCD19⁻ cells from cord blood samples and grown in switch conditions. After 6 weeks, cells collected from individual wells were labeled with PE-CD19 and FITC-CD11b MoAbs and analyzed by flow cytometry. (A) shows the forward scatter and side scatter properties of cells from one representative well and (A2) shows the expression of CD19 and CD11b on the corresponding cells. CD19⁺ cells display a lymphoid morphology (low FSC and SSC) and CD11b⁺ cells have a higher FSC and SSC. (B) and (B2) show the morphologic parameters and simultaneous labeling with CD19 and CD11b of cells collected from one well seeded 6 weeks earlier with a single CD34⁺CD38^low cell sorted from the marrow of an NOD-SCID mouse injected with 20 × 10⁶ CB-MNC.