Fig. 3.
Fig. 3. Effect of RAR-α antagonist on retinoid-induced expression of CD38 mRNA. RAR-α–transduced HL-60R cells were cultured with medium alone (lane 1) or medium containing Ro41-5253 alone (lane 2), ATRA alone (lane 3), ATRA plus Ro-41-5253 (lane 4), 4-oxo RA alone (lane 5), 4-oxo RA plus Ro41-5253 (lane 6), 9-cis RA alone (lane 7), or 9-cis RA plus Ro41-5253 (lane 8). After appropriate treatment (10 nmol/L of each retinoid and/or 1 μmol/L Ro41-5253 for 16 hours), the PCR product specific for CD38 mRNA was generated and resolved by agarose gel electrophoresis. Lane p shows PCR product generated from a plasmid containing the full-length CD38 cDNA sequence. M, DNA molecular weight markers, gl-EcoT141. Lower panel shows equal loading for total RNA used for different samples in RT-PCR.

Effect of RAR-α antagonist on retinoid-induced expression of CD38 mRNA. RAR-α–transduced HL-60R cells were cultured with medium alone (lane 1) or medium containing Ro41-5253 alone (lane 2), ATRA alone (lane 3), ATRA plus Ro-41-5253 (lane 4), 4-oxo RA alone (lane 5), 4-oxo RA plus Ro41-5253 (lane 6), 9-cis RA alone (lane 7), or 9-cis RA plus Ro41-5253 (lane 8). After appropriate treatment (10 nmol/L of each retinoid and/or 1 μmol/L Ro41-5253 for 16 hours), the PCR product specific for CD38 mRNA was generated and resolved by agarose gel electrophoresis. Lane p shows PCR product generated from a plasmid containing the full-length CD38 cDNA sequence. M, DNA molecular weight markers, gl-EcoT141. Lower panel shows equal loading for total RNA used for different samples in RT-PCR.

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