American Society of Hematology

Fig. 4.

$Fig. 4. Downregulation of NADase activity by RAR-α antagonist. The membrane fraction from HL-60 cells cultured in medium alone (control) or medium containing 10 nmol/L of appropriate retinoid without (−) or with (+) 1 μmol/L Ro41-5253, was incubated with 32P-labeled NAD, and the reactants were analyzed by TLC for radioactive products as described in the Materials and Methods. Radioactive bands were either scrapped from the TLC plates, counted by scintillation spectroscopy, and cpms from each lane corresponding to NAD or ADP-ribose were plotted (A), or the plates were developed and the radioactive bands visualized by autoradiography (B).$

Downregulation of NADase activity by RAR-α antagonist. The membrane fraction from HL-60 cells cultured in medium alone (control) or medium containing 10 nmol/L of appropriate retinoid without (−) or with (+) 1 μmol/L Ro41-5253, was incubated with ³²P-labeled NAD, and the reactants were analyzed by TLC for radioactive products as described in the Materials and Methods. Radioactive bands were either scrapped from the TLC plates, counted by scintillation spectroscopy, and cpms from each lane corresponding to NAD or ADP-ribose were plotted (A), or the plates were developed and the radioactive bands visualized by autoradiography (B).

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