IL-10 inhibits IFN-γ–induced ICAM-1 gene transcription. (A) Nuclei were isolated from monocytes stimulated with IFN-γ (100 U/mL) in the presence or absence of IL-10 (100 U/mL) for 3 hours. Transcription occurred in the presence of labeled ribonucleotides. Isolated RNA was hybridized to ICAM-1 cDNA and GAPDH cDNA that was slot blotted onto nitrocellulose. (B) Normalized absorption values were obtained by densitometry scanning of the ICAM-1. From the ratio of ICAM-1 to GAPDH, the fold increase over untreated control cells was calculated. Data are representative of 2 experiments.