Fig. 3.
![Fig. 3. Disulfide-linked receptor complexes in the GM-CSF, IL-3 receptor family. (A) Immunoprecipitation of 125I-surface-labeled primary leukemic cells with anti-βc monoclonal antibody in the absence of ligand or in the presence of IL-3. Proteins were separated on a 7.5% SDS-PAGE gel under nonreducing or reducing (4% 2-mercaptoethanol [2-ME]) conditions. (B) Immunoprecipitation of MO7e cells with anti-βc monoclonal antibody and immunoblotting with antiphosphotyrosine antibody. The cells were either untreated or treated with GM-CSF or IL-3. Proteins were separated on a 7.5% SDS-PAGE gel under non-reducing or reducing (4% 2-ME) conditions.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/5/10.1182_blood.v89.5.1471/3/bl_0050f3.jpeg?Expires=1735500190&Signature=L6UeuV3f~xCwZ6SSpnXJefNnJCFgYlYr~GVzMF1RaciWeLW05VWAP7m6LLgonMiiBJ7J0VRWlP9xP9PTX-ObfOO1j0RsXRELF7L5vv6fJWEw1PkasP5JxaTK6HMtLCDy~wZSzEDcy8bZLTAORoKMH90VE2Au1q7L~0UT7XAZBNDS-ORs1K2XPWNzojwkXWvUlh4huNcXLUPGtRd-fSiChcoN5UfBGu57E30-O1NCYtlq4x5XtfHHPBLgRQ6IBLqHlwyTWaa~n9POOPqOTayZsBpLc4b6ZwYYypLwlcvpI9JZktv6HybCAqNiJDYBOuooJGQb1fw8uBglZYhGKBxYhw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Disulfide-linked receptor complexes in the GM-CSF, IL-3 receptor family. (A) Immunoprecipitation of 125I-surface-labeled primary leukemic cells with anti-βc monoclonal antibody in the absence of ligand or in the presence of IL-3. Proteins were separated on a 7.5% SDS-PAGE gel under nonreducing or reducing (4% 2-mercaptoethanol [2-ME]) conditions. (B) Immunoprecipitation of MO7e cells with anti-βc monoclonal antibody and immunoblotting with antiphosphotyrosine antibody. The cells were either untreated or treated with GM-CSF or IL-3. Proteins were separated on a 7.5% SDS-PAGE gel under non-reducing or reducing (4% 2-ME) conditions.
