Inhibition of leukocyte adhesion by infusion of the anti–P-selectin antibody (WAPS12.2) to the flowing blood. Experimental conditions are described in the legend to Fig 1, except that the infusion solutions also contained anti–P-selectin antibody or the control antibody pl48 (nonblocking antibody directed against the GPIIb subunit) or buffer alone (controls). (A) Leukocyte adhesion to platelet thrombi as determined by morphometrical analysis of cross sections. (B) Leukocyte adhesion to platelet monolayers, quantified on an image analysis system after leukocyte staining. (□), anti-P-selectin antibody WAPS12.2; (○), control antibody pl48. The control value (buffer alone) for platelet thrombi was 42.6 ± 9.8 leukocytes/10 mm cross section, and that for platelet monolayers was 2,570 ± 311 leukocytes/mm2. The values are the mean ± SEM of 4 to 19 coverslips. Comparison with control values (buffer alone) by unpaired Student's t-test: *, P < .02; **, P < .001.
