Phenotype of fresh and cultured, CD1a-enriched CML cells. Freshly isolated PBMNCs from CML patients (A) or immunomagnetically enriched day 10 cells cultured for an additional 4 days (B) were stained with fluorescein- or phycoerythrin-conjugated antibodies and analyzed by flow cytometry. Cellular debris was gated out on the basis of light scatter and the plots depict fluorescence values on all viable cells. The results of one representative experiment are shown. Similar results were noted for seven other patients.