Fig. 3.
Addition of EL-246 eliminated neutrophil rolling (A) and adhesion (data not shown) of DHCB-treated cells. The addition of IB4 (B) at a concentration that entirely inhibited untreated neutrophil adhesion to TNFα-treated endothelium (Fig 1B) was only 60% effective at reducing adhesion of DHCB-treated neutrophils. However, it should be noted that the number of adherent cells was reduced by 30% from untreated cells (Fig 1B) and 60% from DHCB-treated cells (Fig 2B). For (A), (▪) DHCB-treated or (▵) DHCB-treated + EL-246; for (B), (▪) DHCB-treated + IB4 or (▵) untreated + IB4 . *P < .05 relative to DHCB-treated cells. #P < .05 relative to untreated neutrophils + IB4 .
