Fig. 9.
Under static conditions, fibronectin served as ligand for α4 -integrin. Neutrophils adhered avidly to fibronectin and this interaction was not reduced with pretreatment of neutrophils with the anti-CD18 antibody, IB4 . Addition of a fibronectin fragment (FN-40) that contains the CS-1 binding motif for VLA-4 or HP2/1 (anti–α4 -integrin antibody) significantly reduced the adhesive interaction. Under flow conditions, neutrophils were unable to tether to fibronectin (data not shown). (▨) FMLP + DHCB + IB4 ; (▧) FMLP + DHCB + IB4 + FN40; () FMLP + DHCB + IB4 + HP2/1. *P < .05 relative to FMLP + DHCB + IB4 value.
