Gel mobility-shift assay in transfected QT6 cells with GATA transcription factors. (A) Gel mobility-shift assays were performed using 2.5 μg of protein from QT6 cells (lanes 2 and 3), hGATA-1–transfected QT6 cells (lane 4), hGATA-1–transfected QT6 cells under 1% O2 for 24 hours (lane 5), hGATA-2–transfected QT6 cells (lane 6), hGATA-2–transfected QT6 cells under 1% O2 for 24 hours (lane 7), hGATA-3–transfected QT6 cells (lane 8), hGATA-3–transfected QT6 cells under 1% O2 for 24 hours (lane 9), Hep3B cells (lane 10), and Hep3B cells under 1% O2 for 24 hours (lane 11). The position of the complex is noted with a triangle (upper band) and a circle. (B) Gel mobility-shift assay was repeated using competitor for GATA element. A total of 300 ng (6 μL; 150-fold molar excess) of competitor DNA was added to each reaction mixture.
