Fig. 7.
Effect of sense and antisense oligonucleotides for hGATA transcription factors on Hep3B cells. 1 μmol/L or 3 μmol/L of sense and antisense oligonucleotides for hGATA-1, -2, and -3 transcription factors were added to Hep3B cells under 1% O2 for 24 hours without serum. After incubation, the supernatant was assayed for Epo protein by EIA. These results were normalized to the level of Epo protein in Hep3B cells incubated in 1% O2 without oligonucleotide. Bars represent the mean ± 1 SD of four separate experiments. *P < .001 compared with sense (by the Student's t-test).
