The left panel (Transfer Gel) identifies the SDS-PAGE analysis of solubilized membranes of normal controls, patients with HbH, HbH/CS, and patients with homozygous HbCS/CS. Splenectomized patients are indicated by splx. The globin band, running at approximately 15 kD, appears at the bottommost portion of the gel. The arrow indicates bands seen only in HbCS variants that run at a position consistent with 19 kD molecular weight. Western blotting of similar patients is shown on the two righthand panels with a monoclonal anti–α-globin in the center and anti–β-globin MoAb on the right. The anti–β-globin antibody shows accumulations of β-globin in HbH and HbH/CS, but the largest accumulations occurred in splenectomized patients with HbH and HbH/CS. Of importance is the fact that the anti–α-globin antibody reacted specifically with the 19-kD band.