(A) SDS-PAGE analysis of affinity-purified WNT-5a. Total proteins shown by silver-staining (lane 1) and gDWnt5aHis6 detected by Western blotting with the 5B6 MoAb (lane 2). Notice that the prominent 47- to 49-kD band in lane 1 is recognized by the 5B6 MoAb (arrow) and corresponds in size to mature gDWnt5aHis₆ monomers. Other cross-reactive bands in lane 2 correspond to proteolytic products of gDWnt5aHis₆ . (B) Purified WNT-5a promotes the proliferation of flASK cells. Affinity-purified WNT-5a (gDWnt5aHis₆ ) was added to flASK cells in HSC media supplemented with 25 ng/mL KL. The mean cell number was calculated from duplicate wells and repeated in two independent experiments.