Fig. 6.
The effects of anti-Fas antibody on the apoptosis induced in Lin− cells. The mice were inoculated with 1 × 105 PFU of MCMV. On day 3 pi, the mice were killed in order to remove their bone marrow cells. The bone marrow cells were treated with Jo2 antibody for 18 hours. Using flow cytometry, the signals of PI, which represent the DNA contents, were examined after the Lin− populations were gated. Subdiploid cells were designated as cells undergoing apoptosis. The number in the figure represents the percentage of cells undergoing apoptosis in the Lin− cells (mean % ± SEM, n = 4). *Significant increase versus uninfected control (P < .01). †Significant increase versus cells incubated without anti-Fas antibody (P < .01).
