Fig. 7.
The effects of MCMV infection in vitro on the expression of Fas on hematopoietic progenitor cells. After being treated with MCMV for 2 hours at different MOI (multiplicity of infection) values, ranging from 10−2 to 1, bone marrow cells were washed, suspended in RPMI 1640 medium containing 10% FCS, 100 U/mL IL-3, IL-6, and 50 ng/mL stem cell factor and then incubated at 37°C. Forty-eight hours after incubation, the expression level of c-kit and Fas on these cells was examined after the Lin− populations were gated. The numbers in the figure represent the percentage of c-kit+Fas+ cells in the Lin− cell populations.
