Fig. 7.
Agarose gel electrophoresis of 15-PGDH RT-PCR products from total RNA extracted from adult monocytes as described in the Materials and Methods. RT-PCR using primers 15-PGDH 27 and 15-PGDH 850 were performed on RNA from two samples of unstimulated and 1,25-(OH)2D3 –stimulated monocytes after 1 week of culture. Lanes 1 and 2 correspond, respectively, to untreated and treated monocytes (sample 1). Lanes 3 and 4 correspond, respectively, to untreated and treated monocytes (sample 2).
