Agarose gel electrophoresis of COX 2 RT-PCR products from total RNA extracted from neonatal monocytes as described in the Materials and Methods. RT-PCR using primers PGHS-2 1675 and PGHS-2 1868 was performed on RNA from unstimulated and 1,25-(OH)₂D₃ stimulated cells after 24 hours (lanes 1 and 2), 1 week (lanes 3 and 4), 2 weeks (lanes 5 and 6), and 3 weeks of culture (lanes 7 and 8) and on total RNA extracted from HL-60 unstimulated (lane 9) and 1,25-(OH)₂D₃ –stimulated adherent (lane 10) and floating cells (lane 11).