Fig. 1.
Fig. 1. Flow cytometric analysis of PI-stained 3DO (A) and HDK-1 (B) nuclei after 20 hours of in vitro culture in 96-well plates with medium alone, coated with anti-CD3 (1 μg/well) and/or anti-CD2 MoAbs (5 or 1 μg/well; adhered as described in the Materials and Methods). Numbers above histograms indicate the percentage of apoptotic nuclei (broad hypodiploid peak) in a representative experiment. Control AB MoAbs, with the same isotype as anti-CD2 MoAbs, were used as control. PI fluorescence versus the number of nuclei is shown.

Flow cytometric analysis of PI-stained 3DO (A) and HDK-1 (B) nuclei after 20 hours of in vitro culture in 96-well plates with medium alone, coated with anti-CD3 (1 μg/well) and/or anti-CD2 MoAbs (5 or 1 μg/well; adhered as described in the Materials and Methods). Numbers above histograms indicate the percentage of apoptotic nuclei (broad hypodiploid peak) in a representative experiment. Control AB MoAbs, with the same isotype as anti-CD2 MoAbs, were used as control. PI fluorescence versus the number of nuclei is shown.

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