Determination of HLA class I restriction of LMP-specific T-cell clones. LMP-specific clones were isolated by stimulating in vitro PBMC obtained from an HLA-A1,B8 homozygous individual with autologous monocytes infected with vac/LMP1 recombinants and cloning by limiting dilution. Clones were characterized for Class I restriction using a standard CRA. Autologous LCL and allogeneic LCL mismatched and matched at either the A1 or B8 alleles were prepared as previously described. Effector cells were added at an E:T of 10:1. Three representative CTL clones restricted to the B8 allele are shown: SR2F7 (▪), SR4F4 (▨), and SR13F4 ().