Representative LMP2-specific CD8⁺ CTL isolated from a HD patient. Polyclonal lines were generated by stimulating PBMC with autologous LMP2-expressing cells. Responding cells were cloned by limiting dilution in the presence of autologous LCL. Clones were screened for specific lysis of autologous LCL in a standard CRA. Specificity for the LMP2 protein was determined in a CRA by lysis of autologous fibroblasts infected with vac/LMP2, but not cells infected with vac/LMP1 or vac alone. Effector cells were added to the plates at an E:T of 10:1.