Effect of vanadate on growth factorinduced protein tyrosine phosphorylation in PV and normal ECFC. Day-8 cells were incubated with rhEP at 20 U/mL and rhSCF at 100 ng/mL, or medium, for 10 minutes at 37°C after incubation at 37°C with vanadate, 0.4 mmol/L, or medium alone for 1 hour. Cell lysates were immunoprecipitated and immunoblotted with anti-pTyr antibody. The purity of the normal ECFC was 66% ± 8% (A) and 50% ± 6% (B), whereas the purity of the PV ECFC was 63% ± 3% (A) and 47% ± 6% (B).