Fig. 5.
Quantitative RT-PCR analysis of Fas antigen mRNA in myeloma cell lines. Total RNA was extracted from drug-sensitive and drug-resistant cell lines and analyzed for expression of Fas antigen RNA. One hundred nanograms was reverse transcribed, and a 392-bp fragment of the cytoplasmic region of the Fas antigen was amplified using 29 cycles of PCR. PCR products were labeled by the incorporation of α 32P-dCTP in the amplification reaction. (A) Ten percent of the Fas antigen PCR products were separated on a 5% acrylamide gel and quantitated by phosphorimage analysis using ImageQuant software. (B) A 201-bp fragment of histone 3.3 was amplified from the drug-sensitive and -resistant cDNA using 26 cycles of PCR. Lanes are as labeled above.
