Fig. 2.
Binding of 125I-Tpo to normal human platelets. The platelets (1.0 × 108) were incubated with 125I-Tpo (30 pmol/L to 1.2 nmol/L) without (——) or with (⋅⋅⋅) or a 100-fold excess of unlabeled Tpo for 1 hour at 37°C (A). The results represent the mean of triplicate determinations. The data were analyzed using the ligand program (B). Four additional independent experiments gave similar results.