Long-term clonal behavior of engrafted hematopoietic stem cells. Analysis of six long-term reconstituted mice. DNA from CFU-S–derived spleen colonies was cleaved with EcoRI and analyzed by Southern blot. Mice no. 1, 31, and 32 were reconstituted with peripheral blood mononuclears; mice no. 21, 22, and 23 were reconstituted with bone marrow cells. Mouse no. 1, lanes 1 to 3 (clones 10 and 11 on Fig 3) - 6 months; lanes 4 to 11 (clones 28 to 32 on Fig 3) - 9 months; lanes 12 to 20 (clones 38 to 42 on Fig 3) - 12 months after reconstitution. Mouse no. 31, lanes 1 to 3 (clones 1 and 2 on Fig 3) - 3 months; lanes 4 to 11 (clones 2 and 12 to 16 on Fig 3) - 6 months. Mouse no. 32, lanes 1 to 4 (clones 3 and 4 on Fig 3) - 3 months; lanes 5 to 11 (clones 4 and 17 to 19 on Fig 3) - 6 months. Mouse no. 21, lanes 1 to 5 (clones 24 to 28 on Fig 4) - 6 months; lanes 6 to 10 (clones 27 and 54 to 56 on Fig 4) - 9 months; lane 12 (clone 62 on Fig 4 ) - 12 months. Mouse no. 22, lanes 1 to 7 (clones 29 to 33 on Fig 4) - 6 months; lanes 8 to 10 (clones 57 and 58 on Fig 4) - 9 months; lanes 11 to 12 (clones 63 and 64 on Fig 4) - 12 months. Mouse no. 23, lanes 1 and 2 (clones 34 and 35 on Fig 4) - 6 months; lanes 3 to 6 (clones 59 and 60 on Fig 4) - 9 months; lanes 7 to 13 (clones 65 to 69 on Fig 4) - 12 months after reconstitution.