American Society of Hematology

Fig. 5.

$Fig. 5. RP-HPLC chromatograms showing an example of the identification of methylesters of PTH-Glu and PTH-Gla in the N-terminal Gla domain fragments of protein C originating from the patient (fraction 30 in Fig 3). The fragments were subjected to N-terminal sequence analysis after methylation. This enabled the specific detection of Gla and Glu residues and subsequent estimation of the relative amounts of the two residues at potential γ-carboxylation sites (see Table 4). Results from cycles 5 to 9 are shown. Two parallel sequences were identified corresponding to residues 5 to 9 (Leu-Gla-Gla-Leu-Arg) and residues 4 to 8 (Phe-Leu-Gla-Gla-Leu) of the normal and the His extended variant of protein C, respectively. Arrows at the bottom of the figure show the position of the identified residues. X-axis values are minutes and y-axis values are milli absorbance units at 269 nm.$

RP-HPLC chromatograms showing an example of the identification of methylesters of PTH-Glu and PTH-Gla in the N-terminal Gla domain fragments of protein C originating from the patient (fraction 30 in Fig 3). The fragments were subjected to N-terminal sequence analysis after methylation. This enabled the specific detection of Gla and Glu residues and subsequent estimation of the relative amounts of the two residues at potential γ-carboxylation sites (see Table 4). Results from cycles 5 to 9 are shown. Two parallel sequences were identified corresponding to residues 5 to 9 (Leu-Gla-Gla-Leu-Arg) and residues 4 to 8 (Phe-Leu-Gla-Gla-Leu) of the normal and the His extended variant of protein C, respectively. Arrows at the bottom of the figure show the position of the identified residues. X-axis values are minutes and y-axis values are milli absorbance units at 269 nm.

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