Western blotting of membrane PKC in PMA-treated IRBC 5 × 10⁸ RBC or IRBC (70% parasitized) were incubated with or without PMA for 15 minutes and membranes prepared as described in Materials and Methods. Membrane proteins (150 μg/lane) were separated on a 10% acrylamide gel. Lane 1, untreated RBC; lane 2, RBC + PMA; lane 3, untreated IRBC; lane 4, IRBC + PMA. Arrow indicates a band cross-reacting with the PKC-specific antibody MC5. The identity of this band was confirmed by reprobing with specific anti-PKCγ antibody (not shown).