Southern blot analysis of BamHI-, EcoRI-, and Bgl II-digested DNA from patients no. 593 and 975, compared with a placenta negative control (NC). The sizes (in kilobases) of the germline bands are indicated by arrows and the rearranged bands as lines. The 2 faint ≈4- and ≈4.3-kb bands in the BamHI digest from patient no. 593 are probably due to star activity, because they are not seen in other digests.