Fig. 4.
![Fig. 4. Expression of the Tat/Rev antisense gene. At various times following the second reinfusion, PBMC were isolated from the PB of all of the animals. A CD4-enriched cell population was generated by the immunomagnetic depletion of CD8+ cells and placed into culture at 1 × 106 cells/mL in AIM-V + 10% FBS + 200 IU/mL rIL-2. On days 0, 3, and 7 postculture, equal aliquots of cells were removed and total cellular RNA was isolated. RT-PCR reactions were performed using 500 ng of RNA and [32P]-dCTP for detection of the antisense Tat/Rev RNA. Reactions minus the RT were performed as negative controls for this assay. All samples were analyzed on an 8% polyacrylamide gel.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/6/10.1182_blood.v89.6.1987/3/bl_0012f4.jpeg?Expires=1735545071&Signature=prwMgpjr6eh8jEmZzIyq~jAueIL57YM2RiiRSIJMS0ffVLcLv295UZIqvfK4CMhwFFWIpjxzk6TThN3Gi2m~szCHUMWi-2EA2PMT9PJMIDmxNHn0sJcZZChEzPMggP1pk9BOHXRs-hI8JAonvnmzx3jnc0d3RZJm9RpNR9W5MeSZZ66EwAAq-njdKB2dyEC2qAN-ZatRzrKvrU5-qec7z2V2dbKsBnJMxwU82zwRbmDqngsTQ5buPQ4JiTU67Bb51k1c-MNhZFvR~wKdUsgbryUyzAUEIfvgW~ir-Jvke7daGr4mL58qdwjCSzA5DcLcs5hwt73rE1ha6ACPOtkDPA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of the Tat/Rev antisense gene. At various times following the second reinfusion, PBMC were isolated from the PB of all of the animals. A CD4-enriched cell population was generated by the immunomagnetic depletion of CD8+ cells and placed into culture at 1 × 106 cells/mL in AIM-V + 10% FBS + 200 IU/mL rIL-2. On days 0, 3, and 7 postculture, equal aliquots of cells were removed and total cellular RNA was isolated. RT-PCR reactions were performed using 500 ng of RNA and [32P]-dCTP for detection of the antisense Tat/Rev RNA. Reactions minus the RT were performed as negative controls for this assay. All samples were analyzed on an 8% polyacrylamide gel.
