Fig. 2.
Agonist-induced Ca2+ responses in a normal subject. GFP was activated with collagen (C), thrombin (T), or collagen plus thrombin (C + T), with (A) or without (B) stirring, as in Fig 1, and [Ca2+]i measured with fura-2. Typical responses obtained on a normal subject are shown. Initial peak values in controls with thrombin were 952 ± 85 nmol/L (+ stirring) and 807 ± 46 nmol/L (− stirring), and with collagen plus thrombin, the values were 953 ± 85 and 736 ± 58 nmol/L, respectively.