Fig. 3.
Collagen plus thrombin-induced Ca2+ responses. Changes in [Ca2+]i during stimulation of fura-2–loaded GFP with collagen plus thrombin, with (A) or without (B) stirring in 8 normal subjects, 4 patients with thrombasthenia (TSA), and 6 δ-SPD-HPS patients (HPS). The values (mean ± SE) plotted for the first 3 minutes are the mean [Ca2+]i levels at the times indicated, while the values plotted from 4 to 10 minutes are the average [Ca2+]i values measured over the previous minute, calculated as described in Materials and Methods. [Ca2+]i measurements were obtained at 2-second intervals. Agonists were added at 65 seconds.