Induction of FBG γ chain mRNA expression in HepG2 and A549 cells. HepG2 (lanes 1-4) and A549 (lanes 6-9) cells were treated as follows: lanes 1 and 6, no treatment; lanes 2 and 7, 0.1 μmol/L DEX; lanes 3 and 8, 25 U/mL IL-6; lanes 4 and 9, 0.1 μmol/L DEX + 25 U/mL IL-6. (A) Northern blot of 30 μg total RNA from HepG2 or A549 cells, or 5 μg human liver poly(A)⁺ mRNA (lane 5) probed with ³²P-labeled human FBG γ chain cDNA. (B) Duplicate gel of RNA samples stained with acridine orange to confirm the integrity and amount of each RNA sample loaded. Arrowheads mark the 28S and 18S rRNAs.