RT-PCR amplification of FBG Aα, Bβ, and γ chain mRNAs. HepG2 cells (A) and A549 cells (B) were untreated (CON), or treated with DEX, IL-6, or DEX + IL-6. A control PCR reaction with no template was included. Total mRNA was amplified with FBG Aα, Bβ, and γ chain or β-actin primer pairs. The reaction products were resolved on 1.8% agarose gels in TAE buffer. The 100-, 300-, and 500-bp bands of the 100-bp ladder are denoted from top to bottom (arrows).